Stereological and morphological analysis of the effects of aging on spermatogenesis in rat testis 1.

PURPOSE: To quantify, through stereological and morphometric analysis, spermatogenesis in rats undergoing the natural aging process. METHODS: Seventy-two male Wistar rats were divided into 6 equal groups according to age at the time of killing: 3, 6, 9, 12, 18, and 24 months. All the rats were subjected orchiectomy and collection of testicular parenchymal fragments for histological and morphometric analysis. The numerical density of spermatids was calculated using a stereological study, and morphometric analysis was conducted to measure the height of the germinal epithelium and the area of the seminiferous tubules. RESULTS: We found that the 18 and 24 months groups showed a significant reduction in the number of round spermatids. However, the height of the germinal epithelium was not significantly different between the groups. The area of seminiferous tubules was also significantly reduced in the elderly rats compared to that in the young ones. CONCLUSION: Aging of rats showed a significant reduction in the number of round spermatids and the area of the seminiferous tubules, more pronounced in the rats at 18 and 24 months of life.

Stereological and morphological analysis of the effects of aging on spermatogenesis in rat testis

Abstract Purpose: To quantify, through stereological and morphometric analysis, spermatogenesis in rats undergoing the natural aging process. Methods: Seventy-two male Wistar rats were divided into 6 equal groups according to age at the time of killing: 3, 6, 9, 12, 18, and 24 months. All the rats were subjected orchiectomy and collection of testicular parenchymal fragments for histological and morphometric analysis. The numerical density of spermatids was calculated using a stereological study, and morphometric analysis was conducted to measure the height of the germinal epithelium and the area of the seminiferous tubules. Results: We found that the 18 and 24 months groups showed a significant reduction in the number of round spermatids. However, the height of the germinal epithelium was not significantly different between the groups. The area of seminiferous tubules was also significantly reduced in the elderly rats compared to that in the young ones. Conclusion: Aging of rats showed a significant reduction in the number of round spermatids and the area of the seminiferous tubules, more pronounced in the rats at 18 and 24 months of life.

Numeric and volumetric changes in Leydig cells during aging of rats.

PURPOSE: To analyze the effects of aging in rats on the nuclear volume, cytoplasmic volume, and total volume of Leydig cells, as well as their number. METHODS: Seventy-two Wistar rats were divided into six subgroups of 12 rats, which underwent right orchiectomy at 3, 6, 9, 12, 18, and 24 months of age. The weight and volume of the resected testicles were assessed. A stereological study of Leydig cells was conducted, which included measurements of cell number and nuclear, cytoplasmic, and total cell volumes. RESULTS: The weight and volume of the resected testicles showed reductions with age. Only the subgroup composed of 24-month old rats showed a decrease in the nuclear volume of Leydig cells. Significant reductions in the cytoplasmic volume and total volume of Leydig cells were observed in 18- and 24-month old rats. The number of Leydig cells did not vary significantly with age. CONCLUSIONS: Aging in rats resulted in reduction of the nuclear, cytoplasmic, and total cell volumes of Leydig cells. There was no change in the total number of these cells during aging.

Numeric and volumetric changes in Leydig cells during aging of rats

Abstract Purpose: To analyze the effects of aging in rats on the nuclear volume, cytoplasmic volume, and total volume of Leydig cells, as well as their number. Methods: Seventy-two Wistar rats were divided into six subgroups of 12 rats, which underwent right orchiectomy at 3, 6, 9, 12, 18, and 24 months of age. The weight and volume of the resected testicles were assessed. A stereological study of Leydig cells was conducted, which included measurements of cell number and nuclear, cytoplasmic, and total cell volumes. Results: The weight and volume of the resected testicles showed reductions with age. Only the subgroup composed of 24-month old rats showed a decrease in the nuclear volume of Leydig cells. Significant reductions in the cytoplasmic volume and total volume of Leydig cells were observed in 18- and 24-month old rats. The number of Leydig cells did not vary significantly with age. Conclusions: Aging in rats resulted in reduction of the nuclear, cytoplasmic, and total cell volumes of Leydig cells. There was no change in the total number of these cells during aging.

Análise da presença de Helicobacter pylori na secreção da cavidade peritoneal de pacientes com úlcera péptica perfurada admitidos em um hospital terciário / Analysis of the presence of Helicobacter pylori in the secretion of the peritoneal cavity of patients with peptic ulcer perforated admitted in a tertiary hospital

Objetivo: avaliar presença de Helicobacter pylori na secreção peritoneal e no tubo digestivo dos pacientes com úlcera péptica perfurada utilizando teste rápido de urease. Métodos: realizou-se estudo prospectivo, transversal, descritivo, em que se avaliou pacientes atendidos no Setor de Emergência do Hospital Regional Homero de Miranda Gomes de São José (HRHMG), entre fevereiro de 2009 e julho de 2010, portadores de UPP. Coletaram-se, no transoperatório, duas amostras de líquido peritoneal (na proximidade do orifício da úlcera) e outras duas amostras de líquido da cavidade digestiva, uma de cada local sendo encaminhada para cultura e outra injetada em frascos com solução de urease préformada (TUPF). Coletado sorologia para Helicobacter pylori. Resultados: no período do estudo, 14 pacientes foram analisados. A idade média foi de 41,06 anos (DP=13,1). Predominaram pacientes homens (100%), brancos (71,4%), tabagistas (57,2%), IMC < 30 (85,7%), com história prévia de dispepsia (78,6%). O tempo de peritonite pré-operatória foi menor que 24 horas em 85,6% dos casos e o tratamento realizado foi fechamento primário da perfuração com omentoplastia pediculada em todos os pacientes. A sorologia para Helicobacter pylori foi positiva em 84,6% dos pacientes analisados. O TUPF foi positivo em 78,6% das amostras do tubo digestivo e em 42,8% das amostras da cavidade peritoneal. Com relação a estes testes rápidos, 41,6% apresentaram positividade tanto para a amostra do peritônio, quanto para a gástrica/ duodenal, 50% positivaram exclusivamente na amostra da cavidade digestiva e 8,4% exclusivamente na cavidade peritoneal. Dos 11 pacientes que apresentaram sorologia positiva para Helicobacter pylori, 100% apresentaram o TUPF positivo em pelo menos um dos sítios pesquisados. Conclusão: a presença do Helicobacter pylori pode ser avaliada pela realização do teste rápido da urease do fluido coletado em, pelo menos, dois sítios (cavidade peritoneal e cavidade gástrica/duodenal), com igual significância entre os dois métodos. Necessitam maiores estudos para validação deste método.

Objective: to evaluate the presence of Helicobacter pylori in peritoneal secretion and digestive tract of patients with perforated peptic ulcer using urease rapid test d. Methods: we conducted a prospective, cross-sectional, descriptive, which we assessed patients treated at the Emergency Unit of the Hospital Regional de Miranda Gomes Homer of St. Joseph (HRHMG) between February 2009 and July 2010, holders of UPP. Was collected, during surgery, two samples of peritoneal fluid (in the vicinity of the orifice of ulcer) and two samples of fluid from the digestive cavity, one for each location being routed to another culture and injected into vials with a solution of preformed urease (TUPF). Hp collected for serology. Results: during the study period, 14 patients were analyzed. the old me was 41.06 years (SD = 13.1). Most patients were men (100%), white (71.4%), smokers (57.2%), BMI <30 (85.7%), with a history of dyspepsia (78.6%). The time of peritonitis preoperative was less than 24 hours in 85.6% of cases and treatment was fechamendo primary drilling omentoplasty flap from all patients. The H. pylori serology was positive in 84.6% of patients analyzed. The TUPF was positive in 78.6% of the samples of the digestive tract and 42.8% of the samples of the peritoneal cavity. With regard to these rapid tests, 41.6% were positive for both the sample of the peritoneum, as for gastric / duodenal, 50% of the sample only positivaram digestive cavity and only 8.4% in the peritoneal cavity. Of the 11 patients with positive serology for Hp, 100% presented TUPF positive in at least one of the sites surveyed. Conclusion: the presence of Hp can be assessed through the rapid urease test fluid collected in at least two sites (peritoneal cavity and cavity gastric / duodenal), with equal significance between the two methods. Require larger studies to validate this method.

Laparoscopia no tratamento do ureter retrocava: relato de caso e revisão da literatura / Laparoscopy in the treatment of retrocaval ureter: a case report and literature review

É apresentado um caso de ureter retrocava em paciente com queixa de dor lombar à direita, tratada após confirmação por tomografia computadorizada de abdome com contraste intravenoso com transposição do ureter direito (ureteroplastia) associada à implantação de cateter duplo J. sem intercorrências. Dessa forma, enfatiza-se a importância dessa malformação como uma das causas de uropatia obstrutiva e o uso de métodos de imagem para diagnosticá-la.

Tumor adenomatoide epididimário: relato de dois casos e revisão da literatura / Adenomatoid tumor of the epididimys: report a two cases and review the literature

Tumores adenomatóides são tumores mesoteliais benignos geralmente encontrados nas estruturas paratesticulares. Relatamos dois casos de tumor adenomatoide no epididímo. Revisamos a literatura sobre tumores epididimários, suas apresentações, métodos e diagn´soticos e tratamento.

Controle de danos após trauma torácico contuso com lesão cardíaca em criança - relato de caso / damage control after abdominal trauma

O objetivo é descrever o caso de um traumatismo torácico contuso que cursou com grave lesão da artériapulmonar, tipo laceração, que conduziu o paciente ao choque hipovolêmico refratário às medidas de suporte,sendo adotado o controle de danos torácicos. A cirurgia de controle de danos tem sido muito difundida na literatura que aborda trauma, especialmente quando se trata detrauma abdominal. Entretanto, esse relato pretende demonstrar que também é possível adotar a cirurgia de controle de danos quando se aborda um traumatismo torácico grave em pacientes com pouca reserva fisiológica.

The objective is to describe a case of blunt chest trauma causing severe laceration-type injury in the pulmonary artery, which led the patient to developrefractory hypovolemic shock to the support measures, with the adoption of chest damage control. Damage control surgery has been quite spread in the literature on trauma, especially when dealing with abdominal trauma.Nevertheless, this report is aimed to demonstrate that it is also possible to adopt damage control surgery whentreating severe chest trauma in patients with reduced physiological reserve.